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Tutorial for Generating a Phylogenetic Tree:Simplest and Comprehensive guide
As I have explained
earlier in my last video,how to install a MEGA Software.Now moving to the next
step that is generating a phylogenetic tree.So In this video,I am talking about
how to generate a tree using this Software.
Step
wise Representation for generating a Phylogenetic Tree
- Acquiring
the Sequences
MEGA5 opens its own
browser window to show a BLAST page from National Center for Biotechnology
Information (NCBI) from where it gets the sequences.
- Alligning the Sequence
The next step is
allignment of the sequences that we have downloaded from NCBI in FASTA
format.Two methods can be used for Multiple Sequence Allignment :
- Muscle
- CLUSTAL W
- Estimating a PhylogeneticTree
There are several
widely used methods for estimating phylogenetic trees
- Neighbor Joining
- UPGMA
- Bayesian Inference
- Maximum Likelihood ML
- Presenting a Phylogenetic TREE
A drawing of a
phylogenetic tree conveys a lot of information, both explicit and implicit.
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